Coding

Part:BBa_K3588020:Design

Designed by: Louis Gringras   Group: iGEM20_CLS_CLSG_UK   (2020-10-24)


Synthetic Adhesin


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 105
    Illegal PstI site found at 401
    Illegal PstI site found at 649
    Illegal PstI site found at 1096
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 105
    Illegal PstI site found at 401
    Illegal PstI site found at 649
    Illegal PstI site found at 1096
    Illegal NotI site found at 2389
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 105
    Illegal BamHI site found at 1983
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 105
    Illegal PstI site found at 401
    Illegal PstI site found at 649
    Illegal PstI site found at 1096
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 105
    Illegal PstI site found at 401
    Illegal PstI site found at 649
    Illegal PstI site found at 1096
    Illegal NgoMIV site found at 2004
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1244
    Illegal BsaI site found at 2063


Design Notes

ggfh


Source

This Coding region comes from the Spanish department for microbial biotechnology, in their paper titled: "Programming Controlled Adhesion of E. coli to Target Surfaces, Cells, and Tumors with Synthetic Adhesins"

References